Patent Owner: Sobei Genomics, Inc
1. Technical Field
The present invention relates to the field of biomedicine, and more specifically, to the application of using FOXO4-DRI small molecule peptide to remove senescent cells in the medical field.
2. Technical Background
As the human body ages, cells gradually age and their functions decline, which is an important cause of various age-related diseases. How to effectively remove senescent cells, promote cell regeneration, and delay or prevent the aging process is a research focus in the field of biomedicine.
FOXO4 is an important transcription factor that participates in the regulation of various cellular processes, including cell cycle, cell apoptosis, DNA damage repair, etc. Recent studies have shown that FOXO4 is closely related to the aging process. In aging cells, FOXO4 expression increases, and it may affect the aging and apoptosis of cells through interactions with proteins such as p53.
The development of FOXO4-DRI is based on the above background, with the goal of removing aging cells in a relatively safe and targeted way. In animal models, studies have shown that FOXO4-DRI can improve age-related tissue function decline and prolong health life.
3. Invention Content
The present invention provides a method for eliminating senescent cells using a small molecule peptide FOXO4-DRI. FOXO4-DRI is a peptide antagonist that can specifically interfere with the interaction between FOXO4 and p53, thereby changing the intracellular localization of p53 and inducing apoptosis of senescent cells.
Specifically, the FOXO4-DRI small molecule peptide disrupts the interaction between FOXO4 and p53, causing p53 to be excluded from the cell nucleus and directed to the mitochondria. In the mitochondria, p53 induces apoptosis of senescent cells, thereby eliminating senescent cells and promoting cell regeneration.
The present invention also provides a method for using the FOXO4-DRI small molecule peptide for medical treatment, including applying the FOXO4-DRI small molecule peptide to treat diseases related to aging, such as osteoarthritis, cardiovascular disease, neurodegenerative diseases, etc.
IV. Implementation
Example 1: Application of FOXO4-DRI in ACI process
In the process of autologous chondrocyte implantation (ACI), FOXO4-DRI small molecule peptide is applied to the in vitro expansion stage of chondrocytes. By adding FOXO4-DRI to the cell culture medium, senescent chondrocytes can be effectively removed, the activity and quality of implanted cells can be improved, and the therapeutic effect of ACI can be improved.
Example 2: Application of FOXO4-DRI in the treatment of osteoarthritis
In the treatment of osteoarthritis, the FOXO4-DRI small molecule peptide is directly injected into the joint cavity. FOXO4-DRI can effectively remove senescent cells in the joint, reduce joint inflammation, and restore joint function.
5. FOXO4-DRI Study
The Foxo4 peptide was tested in naturally aged animals and in mice genetically engineered to age rapidly. After the mice were put on a harsh chemotherapy regimen, the scientists found some interesting clinical results. In both groups of rats, FOXO4-DRI significantly reduced chemotherapy-induced damage. The FOXO4 drug also restored liver and kidney function as well as fur loss in the animal test subjects.
Neurodegenerative Diseases: Despite extensive research, the exact cause of age-related cognitive decline, such as Alzheimer's, is not fully understood. Changes in proteasome activity have been linked to neurodegenerative diseases. Disrupted proteasome activity has been linked to Parkinson's disease, dementia praecox, Huntington's disease, and prion diseases. However, it is unclear whether this is a primary or secondary cause.
Additionally, ALS (Lou Gehrig's disease) also affects proteasome function. The discovery that extracellular FOXO proteins may help treat or prevent neurodegenerative diseases has prompted researchers to explore the idea that exogenous FOXO proteins may help. The modified FOXO protein FOXO4-DRI may at least help slow the progression of neurodegenerative diseases.
Heart disease: Older people are more susceptible to heart disease because of a decrease in the activity of the heart's proteasomes. Proteasomes are responsible for clearing away proteins that cells have marked as "damaged" or not working properly. Studies in rats have shown that aging affects the activity of proteasomes, resulting in more damaged proteins in the heart.
FOXO proteins contribute to autophagy and the activity of the proteasome. Increased levels of FOXO4 lead to increased proteasome activity, which reduces oxidation and protein damage in specific tissues. FOXO4-DRI or its variants may contribute to the heart's natural housekeeping functions, which may help to mitigate the effects of aging on the heart's ability to pump blood throughout the body.
Anti-aging: Blocking or senescent cells benefits from FOXO4. Because it attaches to p53, it can prevent senescent cells from dying. DRI peptide blocks FOXO4-p53 interaction. P53 activates apoptotic proteins and causes cell death. By removing old cells from tissues, more significant tissue regeneration can be achieved. In studies, DRI peptide promoted tissue regeneration and extended lifespan in aging mice.
This is the period of physical activity. It decreases with age. By reducing biological tissue aging, a person's healthy life span can be extended. Older mice live longer, are more active, and have fewer injuries such as heart disease and musculoskeletal problems.
6. How to use FOXO4-DRI small molecule peptide
Proper use of Foxo 4 in research requires careful handling and administration of the peptide. Researchers should ensure that the peptide is properly stored to maintain its stability and effectiveness. When administering the peptide to animal models, it is important to use aseptic technique to avoid contamination. In addition, the dose should be carefully calculated and adjusted if necessary based on the response of the animal model.
How to Mix Foxo 4: When preparing Foxo 4 for research applications, it is important to follow the correct mixing procedure. The peptide should be reconstituted using a suitable solvent (e.g., bacteriostatic water). The mixture should be gently stirred until the peptide is completely dissolved. It is critical to maintain a sterile environment throughout the process to prevent contamination.
Best Foxo 4 Results: Some of the most compelling research findings involving Foxo 4 peptides include their potential role in regulating cell cycle progression and apoptosis, their ability to enhance cellular resistance to oxidative stress, and their potential applications in cancer and aging research. These findings highlight the potential of Foxo 4 peptides for therapeutic applications and further research.
Foxo 4 Research Topic: Foxo 4's effectiveness in solving specific situations
Studies have shown that Foxo 4 peptides can effectively address a variety of pathologies in non-human models. For example, studies have shown that Foxo 4 can promote cell cycle arrest and apoptosis, thereby significantly reducing tumor growth in certain cancer models. In addition, Foxo 4 peptides have been shown to enhance cellular resistance to oxidative stress, suggesting potential applications in the study of aging and neurodegenerative diseases.
The role of Foxo 4 peptide in cell and molecular biology research
Foxo 4 peptides have been widely used in cell and molecular biology research. They are known to play crucial roles in regulating various biological processes, including cell cycle progression, apoptosis, and resistance to oxidative stress. This makes them valuable tools for studying the cellular and molecular mechanisms behind these processes.
Potential of Foxo 4 Peptide in Drug Development
Foxo 4-based therapies are currently being explored for their therapeutic potential in a variety of medical conditions. Given their role in regulating cell cycle progression and apoptosis, Foxo 4 peptides have the potential to be used in the development of novel cancer therapies. In addition, their ability to enhance cellular resistance to oxidative stress suggests potential applications in the treatment of aging and neurodegenerative diseases.
Synthesis and Modification of Foxo 4 Peptides: Ongoing research efforts are focused on synthesizing and modifying Foxo 4 peptides to create more potent and stable versions. These efforts involve a variety of techniques, including peptide synthesis and protein engineering. The goal is to enhance the therapeutic potential of Foxo 4 peptides while minimizing any potential side effects.
What Foxo 4 is used for: Foxo 4 peptides are primarily used to study various biological processes, including cell cycle progression, apoptosis, and oxidative stress resistance. They are also used to develop novel therapeutic strategies for various medical conditions, including cancer and neurodegenerative diseases.
Future research directions for Foxo 4: Potential future research directions for Foxo 4 include exploring new therapeutic applications, further studying its potential mechanisms of action, and developing optimized Foxo 4 analogs. These efforts may lead to the development of novel Foxo 4-based therapies for a variety of medical conditions.
Foxo 4 is usually given by injection, which allows for precise control of dosage.
VII. Effect Evaluation
Through a series of experiments and clinical studies, we found that the use of FOXO4-DRI small molecule peptide can effectively remove senescent cells, increase cell activity, and improve disease symptoms. The use of FOXO4-DRI in the ACI process can significantly improve the implantation effect of chondrocytes and promote the recovery of joint function. In the treatment of osteoarthritis, FOXO4-DRI can significantly reduce joint inflammation and improve the quality of life of patients.
8. Rights Claims
1. A method for eliminating senescent cells using FOXO4-DRI small molecule peptide, characterized in that the apoptosis of senescent cells is induced by interfering with the interaction between FOXO4 and p53.
2. A method for medical treatment using a FOXO4-DRI small molecule peptide, characterized in that the FOXO4-DRI small molecule peptide is used to treat diseases related to aging.
3. A method for using FOXO4-DRI small molecule peptide in autologous chondrocyte implantation (ACI), characterized in that FOXO4-DRI is added to the cell culture medium to remove senescent chondrocytes.
The present invention provides a new anti-aging strategy for the biomedical field and offers new possibilities for treating diseases related to aging.